BRCA1 antibody (N-Term)

Catalog No. ABIN2779333

The Rabbit Polyclonal anti-BRCA1 antibody is suitable to detect BRCA1 in samples from Human, Rat, Horse, Dog and Rabbit. It has been validated for WB.

Quick Overview for BRCA1 antibody (N-Term) (ABIN2779333)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human, Rat, Horse, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRCA1 Antibody

Binding Specificity: N-Term

Purpose: BRCA1 Antibody - N-terminal region

Sequence: MDLSALRVEE VQNVINAMQK ILECPICLEL IKEPVSTKCD HIFCKFCMLK

Predicted Reactivity: Dog: 92%, Horse: 85%, Human: 100%, Rabbit: 85%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against BRCA1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BRCA1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30434-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background:

Background Information: The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.

Gene Name: Breast cancer 1, early onset

Alternative Symbols: IRIS, PSCP, BRCAI, BRCC1, FANCS, PNCA4, RNF53, BROVCA1, PPP1R53

Protein Name: Breast cancer type 1 susceptibility protein

Molecular Weight: 76kDa

Gene ID: 672

NCBI Accession: NP_009229

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus