RAD17 antibody (N-Term)

Catalog No. ABIN2779342

This Rabbit Polyclonal antibody specifically detects RAD17 in WB. It exhibits reactivity toward Human, Rat, Dog, Cow, Pig, Horse and Rabbit.

Quick Overview for RAD17 antibody (N-Term) (ABIN2779342)

Target: RAD17

Reactivity: Human, Rat, Dog, Cow, Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAD17 Antibody

Binding Specificity: N-Term

Purpose: RAD17 Antibody - N-terminal region

Sequence: MNQVTDWVDP SFDDFLECSG VSTITATSLG VNNSSHRRKN GPSTLESSRF

Predicted Reactivity: Cow: 89%, Dog: 100%, Horse: 92%, Human: 100%, Pig: 100%, Rabbit: 100%, Rat: 89%

Characteristics: This is a rabbit polyclonal antibody against RAD17. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RAD17

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30546-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAD17

Target: RAD17

Alternative Name: RAD17

Background:

Background Information: RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.

Gene Name: RAD17 homolog (S. pombe)

Alternative Symbols: CCYC, R24L, RAD24, HRAD17, RAD17SP

Protein Name: Cell cycle checkpoint protein RAD17

Molecular Weight: 76kDa

Gene ID: 5884

NCBI Accession: NP_002864

UniProt: O75943