MAP3K8 antibody (C-Term)

Catalog No. ABIN2779365

The Rabbit Polyclonal anti-MAP3K8 antibody (ABIN2779365) specifically detects MAP3K8 in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Horse, Dog, Rabbit and Guinea Pig samples.

Quick Overview for MAP3K8 antibody (C-Term) (ABIN2779365)

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP3K8 Antibody

Binding Specificity: C-Term

Purpose: MAP3K8 Antibody - C-terminal region

Sequence: PRCQSLDSAL LERKRLLSRK ELELPENIAD SSCTGSTEES EMLKRQRSLY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 77%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MAP3K8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MAP3K8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8105675

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MAP3K8

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Alternative Name: MAP3K8

Background:

Background Information: MAP3K8 is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.

Gene Name: Mitogen-activated protein kinase kinase kinase 8

Alternative Symbols: COT, EST, ESTF, TPL2, AURA2, MEKK8, Tpl-2, c-COT

Protein Name: Mitogen-activated protein kinase kinase kinase 8

Molecular Weight: 53kDa

Gene ID: 1326

NCBI Accession: NP_005195

UniProt: P41279

Pathways: PI3K-Akt Signaling, TCR Signaling