Nkx2-2 antibody (N-Term)

Catalog No. ABIN2779437

The Rabbit Polyclonal anti-Nkx2-2 antibody is suitable to detect Nkx2-2 in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Sheep and Pig. It has been validated for WB.

Quick Overview for Nkx2-2 antibody (N-Term) (ABIN2779437)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Sheep, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nkx2-2 Antibody

Binding Specificity: N-Term

Purpose: NKX2-2 Antibody - N-terminal region

Sequence: GLAAGAPPQD SSSKSPEPSA DESPDNDKET PGGGGDAGKK RKRRVLFSKA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against NKX2-2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human NKX2-2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32044-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2-2

Background:

Background Information: Nkx2-2 contains 1 homeobox DNA-binding domain which is essential for interaction with OLIG2. Nkx2-2 may be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.

Gene Name: NK2 homeobox 2

Alternative Symbols: NKX2B, NKX2.2

Protein Name: Homeobox protein Nkx-2.2

Molecular Weight: 30kDa

Gene ID: 4821

NCBI Accession: NP_002500

UniProt: O95096

Pathways: Dopaminergic Neurogenesis