WNT5A antibody (Middle Region)

Catalog No. ABIN2779470

This Rabbit Polyclonal antibody specifically detects WNT5A in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse and Zebrafish (Danio rerio).

Quick Overview for WNT5A antibody (Middle Region) (ABIN2779470)

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-WNT5A Antibody

Binding Specificity: Middle Region

Sequence: GGCGDNIDYG YRFAKEFVDA RERERIHAKG SYESARILMN LHNNEAGRRT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 87%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 80%

Characteristics: This is a rabbit polyclonal antibody against WNT5A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human WNT5A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 380 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for WNT5A

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Alternative Name: WNT5A

Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT5A is a member of the WNT protein family. It shows 98 % , 98 % and 87 % amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98 %, 98 % and 87 % amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: hWNT5A
Protein Interaction Partner: UBC, LRP6, FZD2, FZD1, ROR2, FZD5,
Protein Size: 380

Molecular Weight: 42 kDa

Gene ID: 7474

NCBI Accession: NM_003392, NP_003383

UniProt: P41221

Pathways: WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation