ESR2 antibody (Middle Region)

Catalog No. ABIN2779528

The Rabbit Polyclonal anti-ESR2 antibody (ABIN2779528) specifically detects ESR2 in WB. The antibody is reactive with Human, Mouse, Rat, Pig, Sheep, Horse, Dog, Cow, Rabbit, Guinea Pig and Goat samples.

Quick Overview for ESR2 antibody (Middle Region) (ABIN2779528)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human, Mouse, Rat, Pig, Sheep, Horse, Dog, Cow, Rabbit, Guinea Pig, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ESR2 Antibody

Binding Specificity: Middle Region

Purpose: ESR2 Antibody - middle region

Sequence: EYLCVKAMIL LNSSMYPLVT ATQDADSSRK LAHLLNAVTD ALVWVIAKSG

Predicted Reactivity: Cow: 86%, Dog: 86%, Goat: 86%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 86%, Pig: 86%, Rabbit: 86%, Rat: 86%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against ESR2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ESR2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32258-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: ESR2

Background:

Background Information: ESR2 is a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. It contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.

Gene Name: Estrogen receptor 2 (ER beta)

Alternative Symbols: Erb, ESRB, ODG8, ESTRB, NR3A2, ER-BETA, ESR-BETA

Protein Name: Estrogen receptor beta

Molecular Weight: 59kDa

Gene ID: 2100

NCBI Accession: NP_001428

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling