TLE2 antibody (N-Term)

Catalog No. ABIN2779582

Product Details anti-TLE2 Antibody

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VEEERPSGPG GGGKQRADEK EPSGPYESDE DKSDYNLVVD EDQPSEPPSP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TLE2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TLE2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 743 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TLE2

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Alternative Name: TLE2 (TLE2 Products)

Synonyms: TLE2 antibody, ESG antibody, ESG2 antibody, GRG2 antibody, Grg2 antibody, mKIAA4188 antibody, transducin like enhancer of split 2 antibody, transducin-like enhancer of split 2 antibody, TLE2 antibody, Tle2 antibody

Background: TLE2 belongs to the WD repeat Groucho/TLE family. It contains 6 WD repeats. TLE2 is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
Alias Symbols: ESG, ESG2, FLJ41188, GRG2
Protein Interaction Partner: UBC, SUMO1, ORF50, LEF1, BARHL1, TLX3, VENTX, CUL4A, CUL4B, PAX9, MSX2, MSX1, FOXA3, EMX2, PRDM1, H3F3B, TLE1, UTY, HMGB1, RUNX1, TLE2, HES1,
Protein Size: 743

Molecular Weight: 80 kDa

Gene ID: 7089

NCBI Accession: NM_003260, NP_003251

UniProt: Q04725

Pathways: WNT Signaling