POLR2B antibody (N-Term)

Catalog No. ABIN2779657

This Rabbit Polyclonal antibody specifically detects POLR2B in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse and Rabbit.

Quick Overview for POLR2B antibody (N-Term) (ABIN2779657)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2B Antibody

Binding Specificity: N-Term

Purpose: POLR2B Antibody - N-terminal region

Sequence: TYSAPLYVDI TKTVIKEGEE QLQTQHQKTF IGKIPIMLRS TYCLLNGLTD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against POLR2B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLR2B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108543

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Alternative Name: POLR2B

Background:

Targeted Subunit: RPB2

Background Information: POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Gene Name: Polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa

Alternative Symbols: RPB2, POL2RB, hRPB140

Protein Name: DNA-directed RNA polymerase II subunit RPB2

Molecular Weight: 134kDa

Gene ID: 5431

NCBI Accession: NP_000929

UniProt: P30876

Pathways: Regulatory RNA Pathways, DNA Damage Repair