NCOA3 antibody (Middle Region)

Catalog No. ABIN2779677

This Rabbit Polyclonal antibody specifically detects NCOA3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse and Pig.

Quick Overview for NCOA3 antibody (Middle Region) (ABIN2779677)

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NCOA3 Antibody

Binding Specificity: Middle Region

Purpose: NCOA3 Antibody - middle region

Sequence: DQNPVESSMC QSNSRDHLSD KESKESSVEG AENQRGPLES KGHKKLLQLL

Predicted Reactivity: Cow: 77%, Dog: 90%, Horse: 92%, Human: 100%, Mouse: 83%, Pig: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NCOA3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NCOA3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106770

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Alternative Name: NCOA3

Background:

Background Information: NCOA3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

Gene Name: Nuclear receptor coactivator 3

Alternative Symbols: ACTR, AIB1, RAC3, SRC3, pCIP, AIB-1, CTG26, SRC-3, CAGH16, KAT13B, TNRC14, TNRC16, TRAM-1, bHLHe42

Protein Name: Nuclear receptor coactivator 3

Molecular Weight: 155kDa

Gene ID: 8202

NCBI Accession: NP_006525

UniProt: Q0VF45

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha