CHEK1 antibody (Middle Region)

Catalog No. ABIN2779737

The Rabbit Polyclonal anti-CHEK1 antibody has been validated for WB and IHC. It is suitable to detect CHEK1 in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Horse and Guinea Pig.

Quick Overview for CHEK1 antibody (Middle Region) (ABIN2779737)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CHEK1 Antibody

Binding Specificity: Middle Region

Sequence: SARITIPDIK KDRWYNKPLK KGAKRPRVTS GGVSESPSGF SKHIQSNLDF

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CHEK1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CHEK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 476 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHEK1

Background: CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Alias Symbols: CHK1
Protein Interaction Partner: RELA, CUL4A, UBC, SETMAR, DDB1, TNRC6B, ATR, HECW2, CLSPN, DTL, BTG3, TOPBP1, MAPKAPK2, HSP90AA1, CDC25C, RAD51, PIGM, CUL1, VCP, CDC25A, ATM, APP, MED15, NEK2, TLK1, RB1, BRCA1, FEM1B, XIAP, COPS5, MEPE, LATS2, TP53, Chek1, MEN1, USP7, DNMT1, FANCE, PCNA
Protein Size: 476

Molecular Weight: 54 kDa

Gene ID: 1111

NCBI Accession: NM_001274, NP_001265

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair