MDC1 antibody (C-Term)

Catalog No. ABIN2779807

Product Details anti-MDC1 Antibody

Target: MDC1 (Mdc1 (MDC1))

Binding Specificity: C-Term

Reactivity: Human, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDC1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GKEEDVVTPK PGKRKRDQAE EEPNRIPSRS LRRTKLNQES TAPKVLFTGV

Predicted Reactivity: Dog: 86%, Guinea Pig: 92%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against MDC1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MDC1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 2089 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MDC1

Target: MDC1 (Mdc1 (MDC1))

Alternative Name: MDC1 (MDC1 Products)

Synonyms: 6820401C03 antibody, AA413496 antibody, Nfbd1 antibody, mKIAA0170 antibody, NFBD1 antibody, mediator of DNA damage checkpoint 1 antibody, mediator of DNA-damage checkpoint 1 antibody, Mdc1 antibody, MDC1 antibody

Background: The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
Alias Symbols: DKFZp781A0122, KIAA0170, MGC166888, NFBD1
Protein Interaction Partner: RPA3, RPA2, RPA1, EED, TOPBP1, RNF8, UBC, LMNA, RAG1, CDC27, NCL, NBN, MRE11A, CSNK2A1, RAD50, CAPZB, CAPZA1, AUP1, ATP2A2, LOC729324, CHAMP1, HNRNPUL2, PGAM5, LSM12, ANAPC16, OSBPL8, DTX2, ZNF251, RBM17, FAM175A, LAS1L, LPCAT1, MYH14, SLC25A22, ZNF768, H
Protein Size: 2089

Molecular Weight: 227 kDa

Gene ID: 9656

NCBI Accession: NM_014641, NP_055456

UniProt: Q14676