MDC1 antibody (C-Term)

Catalog No. ABIN2779807

The Rabbit Polyclonal anti-MDC1 antibody is suitable to detect MDC1 in samples from Human, Dog and Guinea Pig. It has been validated for WB.

Quick Overview for MDC1 antibody (C-Term) (ABIN2779807)

Target: MDC1 (Mdc1 (MDC1))

Reactivity: Human, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MDC1 Antibody

Binding Specificity: C-Term

Purpose: MDC1 Antibody - C-terminal region

Sequence: GKEEDVVTPK PGKRKRDQAE EEPNRIPSRS LRRTKLNQES TAPKVLFTGV

Predicted Reactivity: Dog: 86%, Guinea Pig: 92%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against MDC1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MDC1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36798-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MDC1

Target: MDC1 (Mdc1 (MDC1))

Alternative Name: MDC1

Background:

Background Information: The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Gene Name: Mediator of DNA-damage checkpoint 1

Alternative Symbols: NFBD1

Protein Name: Mediator of DNA damage checkpoint protein 1

Molecular Weight: 227kDa

Gene ID: 9656

NCBI Accession: NP_055456

UniProt: Q14676