ECSIT antibody (N-Term)

Catalog No. ABIN2779983

This Rabbit Polyclonal antibody specifically detects ECSIT in WB. It exhibits reactivity toward Mouse.

Quick Overview for ECSIT antibody (N-Term) (ABIN2779983)

Target: ECSIT

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECSIT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ECSIT Antibody

Binding Specificity: N-Term

Purpose: Ecsit Antibody - N-terminal region

Sequence: EPWLVPRPPE PQRKPIKVPA MHEDSFKPSG NRERDKASFL NAVRSFGAHN

Predicted Reactivity: Mouse: 100%

Characteristics: This is a rabbit polyclonal antibody against Ecsit. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP37175-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ECSIT

Target: ECSIT

Alternative Name: Ecsit

Background:

Background Information: Ecsit is required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. Ecsit is an adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1.Ecsit promotes proteolytic activation of MAP3K1. Ecsit is involved in the BMP signaling pathway. Ecsit is required for normal embryonic development.

Gene Name: ECSIT homolog (Drosophila)

Alternative Symbols: Sit, Sitpec

Protein Name: Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial

Molecular Weight: 50kDa

Gene ID: 26940

NCBI Accession: NP_036159

UniProt: Q9QZH6

Pathways: TLR Signaling, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome