PYCARD antibody (Middle Region)

Catalog No. ABIN183475

This Rabbit Polyclonal antibody specifically detects PYCARD in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for PYCARD antibody (Middle Region) (ABIN183475)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PYCARD Antibody

Binding Specificity: Middle Region

Sequence: TVLRDMGLQE LAEQLQTTKE ESGAVAAAAS VPAQSTARTG HFVDQHRQAL

Predicted Reactivity: Mouse: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against PYCARD. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse PYCARD

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 193 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: PYCARD

Background: Pycard is a cytosolic soluble protein that forms insoluble aggregates and enhances etoposide-induced apoptosis.
Alias Symbols: Asc, TNS1, masc, CARD5, TMS-1, 9130417A21Rik
Protein Size: 193

Molecular Weight: 21 kDa

Gene ID: 66824

NCBI Accession: NM_023258, NP_075747

UniProt: Q9EPB4

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome