Estrogen Receptor alpha antibody (N-Term)

Catalog No. ABIN501619

This Rabbit Polyclonal antibody specifically detects Estrogen Receptor alpha in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Goat and Guinea Pig.

Quick Overview for Estrogen Receptor alpha antibody (N-Term) (ABIN501619)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Goat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: N-Term

Sequence: LERPLGEVYL DSSKPAVYNY PEGAAYEFNA AAAANAQVYG QTGLPYGPGS

Predicted Reactivity: Cow: 93%, Dog: 79%, Goat: 93%, Guinea Pig: 93%, Horse: 79%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against ESR1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ESR1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 595 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1

Background: ESR1 is an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodi
Alias Symbols: DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1
Protein Interaction Partner: SRC, DDX5, POLR2C, POLR2B, POLR2A, MSH2, TTC5, KAT5, NCOA2, BRCA1, MED24, UBC, PSMB9, MED1, POLR2L, POLR2K, POLR2J, POLR2I, POLR2H, POLR2G, POLR2F, POLR2E, POLR2D, ASCC1, TRRAP, PHB2, NCOR1, NCOA1, PIK3CA, IGF1R, HDAC1, NSD1, WIPI1, ESR1, ZNF131, RANBP9,
Protein Size: 595

Molecular Weight: 66 kDa

Gene ID: 2099

NCBI Accession: NM_000125, NP_000116

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly