HMGB1 antibody (N-Term)

Catalog No. ABIN501641

There is 1 publication for this product available. The Rabbit Polyclonal anti-HMGB1 antibody is suitable to detect HMGB1 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Rabbit. It has been validated for WB.

Quick Overview for HMGB1 antibody (N-Term) (ABIN501641)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HMGB1 Antibody

Binding Specificity: N-Term

Purpose: HMGB1 Antibody - N-terminal region

Sequence: GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HMGB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HMGB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38109-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HMGB1 antibody (ABIN501641)

Straino, Di Carlo, Mangoni, De Mori, Guerra, Maurelli, Panacchia, Di Giacomo, Palumbo, Di Campli, Uccioli, Biglioli, Bianchi, Capogrossi, Germani: "High-mobility group box 1 protein in human and murine skin: involvement in wound healing." in: The Journal of investigative dermatology, Vol. 128, Issue 6, pp. 1545-53, (2008) (PubMed).

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background:

Background Information: HMGB1 belongs to the HMGB family. It contains 2 HMG box DNA-binding domains. The protein binds preferentially single-stranded DNA and unwinds double stranded DNA. The oxidized HMGB1 may accumulate even in cells under oxidative stress.

Gene Name: High mobility group box 1

Alternative Symbols: HMG1, HMG3, HMG-1, SBP-1

Protein Name: High mobility group protein B1

Molecular Weight: 25kDa

Gene ID: 3146

NCBI Accession: NP_002119

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome