Cyclin B1 antibody (C-Term)

Catalog No. ABIN2781485

The Rabbit Polyclonal anti-Cyclin B1 antibody is suitable to detect Cyclin B1 in samples from Human, Rat, Cow, Pig, Dog, Horse, Rabbit and Guinea Pig. It has been validated for WB.

Quick Overview for Cyclin B1 antibody (C-Term) (ABIN2781485)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Rat, Cow, Pig, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cyclin B1 Antibody

Binding Specificity: C-Term

Purpose: CCNB1 Antibody - C-terminal region

Sequence: AKNVVMVNQG LTKHMTVKNK YATSKHAKIS TLPQLNSALV QDLAKAVAKV

Predicted Reactivity: Cow: 95%, Dog: 95%, Guinea Pig: 85%, Horse: 90%, Human: 100%, Pig: 95%, Rabbit: 95%, Rat: 80%

Characteristics: This is a rabbit polyclonal antibody against CCNB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CCNB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43600-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1

Background:

Background Information: CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Cyclin B1

Alternative Symbols: CCNB

Protein Name: G2/mitotic-specific cyclin-B1

Molecular Weight: 48kDa

Gene ID: 891

NCBI Accession: NP_114172

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase