EPO antibody (Middle Region)

Catalog No. ABIN2781825

This Rabbit Polyclonal antibody specifically detects EPO in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit and Sheep.

Quick Overview for EPO antibody (Middle Region) (ABIN2781825)

Target: EPO (Erythropoietin (EPO))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPO antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EPO Antibody

Binding Specificity: Middle Region

Purpose: EPO Antibody - middle region

Sequence: KEAISPPDAA SAAPLRTITA DTFRKLFRVY SNFLRGKLKL YTGEACRTGD

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against EPO. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EPO

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44337-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO

Target Type: Hormone

Background:

Background Information: This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Gene Name: Erythropoietin

Alternative Symbols: EP, DBAL, ECYT5, MVCD2

Protein Name: Erythropoietin

Molecular Weight: 18kDa

Gene ID: 2056

NCBI Accession: NP_000790

UniProt: P01588

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity