Estrogen Receptor alpha antibody (Middle Region)

Catalog No. ABIN2782261

This Rabbit Polyclonal antibody specifically detects Estrogen Receptor alpha in WB. It exhibits reactivity toward Human, Rat, Cow, Pig, Dog and Sheep.

Quick Overview for Estrogen Receptor alpha antibody (Middle Region) (ABIN2782261)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Rat, Cow, Pig, Dog, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: Middle Region

Purpose: ESR1 Antibody - middle region

Sequence: LLEMLDAHRL HAPTSRGGAS VEETDQSHLA TAGSTSSHSL QKYYITGEAE

Predicted Reactivity: Cow: 83%, Dog: 79%, Human: 100%, Pig: 79%, Rat: 86%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against ESR1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ESR1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP45592-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1

Background:

Background Information: Hairy/enhancer of split-related proteins, such as HEY1, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.

Gene Name: Estrogen receptor 1

Alternative Symbols: ER, ESR, Era, ESRA, ESTRR, NR3A1

Protein Name: Estrogen receptor

Molecular Weight: 66kDa

Gene ID: 2099

NCBI Accession: NP_000116

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly