HAL antibody (N-Term)

Catalog No. ABIN2782320

Cited in 1 publication. The Rabbit Polyclonal anti-HAL antibody (ABIN2782320) specifically detects HAL in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Rabbit, Dog, Guinea Pig, Horse and Pig samples.

Quick Overview for HAL antibody (N-Term) (ABIN2782320)

Target: HAL (Histidine Ammonia-Lyase (HAL))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Rabbit, Dog, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HAL Antibody

Binding Specificity: N-Term

Purpose: HAL Antibody - N-terminal region

Sequence: INKLQELQVN LVRSHSSGVG KPLSPERCRM LLALRINVLA KGYSGISLET

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against HAL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HAL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP45692-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HAL antibody (ABIN2782320)

Alemán, Ortíz, Langley, Tovar, Torres: "Regulation by glucagon of the rat histidase gene promoter in cultured rat hepatocytes and human hepatoblastoma cells." in: American journal of physiology. Endocrinology and metabolism, Vol. 289, Issue 1, pp. E172-9, (2005) (PubMed).

Target Details for HAL

Target: HAL (Histidine Ammonia-Lyase (HAL))

Alternative Name: HAL

Background:

Background Information: HAL is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. HAL defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids.

Gene Name: Histidine ammonia-lyase

Alternative Symbols: HIS, HSTD

Protein Name: Histidine ammonia-lyase

Molecular Weight: 73 kDa

Gene ID: 3034

NCBI Accession: NP_002099

UniProt: P42357