FEN1 antibody (N-Term)

Catalog No. ABIN2782482

The Rabbit Polyclonal anti-FEN1 antibody is suitable to detect FEN1 in samples from Human, Mouse, Rat, Cow, Pig, Sheep, Horse, Dog, Guinea Pig, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for FEN1 antibody (N-Term) (ABIN2782482)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Sheep, Horse, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FEN1 Antibody

Binding Specificity: N-Term

Purpose: FEN1 Antibody - N-terminal region

Sequence: APSAIRENDI KSYFGRKVAI DASMSIYQFL IAVRQGGDVL QNEEGETTSH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against FEN1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46096-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN1

Background:

Background Information: FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Gene Name: Flap structure-specific endonuclease 1

Alternative Symbols: MF1, RAD2, FEN-1

Protein Name: Flap endonuclease 1

Molecular Weight: 42kDa

Gene ID: 2237

NCBI Accession: NP_004102

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA