PLK1 antibody (C-Term)

Catalog No. ABIN2782496

This Rabbit Polyclonal antibody specifically detects PLK1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig and Horse.

Quick Overview for PLK1 antibody (C-Term) (ABIN2782496)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PLK1 Antibody

Binding Specificity: C-Term

Sequence: KRDFRTYRLS LLEEYGCCKE LASRLRYART MVDKLLSSRS ASNRLKAS

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against PLK1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PLK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 603 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.
Alias Symbols: PLK, STPK13
Protein Interaction Partner: RAP1GAP, CHFR, KLHL22, BRCA1, BORA, EGFR, UBC, NUAK1, MUS81, FBXO6, PPP6R2, STK3, BUB1B, FZR1, CDC20, CHEK2, TP53BP1, CDCA5, LATS1, CENPU, CDC25C, KLHL21, OFD1, CUL2, Sugt1, KRBA1, HSPA4, PHC2, KLHL9, FLJ20712, RABAC1, TANK, RBX1, VCP, CDH1, APP, STAG2, N
Protein Size: 603

Molecular Weight: 68 kDa

Gene ID: 5347

NCBI Accession: NM_005030, NP_005021

UniProt: P53350

Pathways: Cell Division Cycle, M Phase