MTHFD1 antibody (Middle Region)

Catalog No. ABIN2782515

Product Details anti-MTHFD1 Antibody

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Horse, Zebrafish (Danio rerio), Pig, Dog, Guinea Pig, Cow, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTHFD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: CMAKTHLSLS HNPEQKGVPT GFILPIRDIR ASVGAGFLYP LVGTMSTMPG

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against MTHFD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MTHFD1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 935 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MTHFD1

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Alternative Name: MTHFD1 (MTHFD1 Products)

Synonyms: MTHFC antibody, MTHFD antibody, Tb07.27M11.970 antibody, Dcs antibody, E430024A07Rik antibody, Mthfd antibody, GB13160 antibody, cb325 antibody, fc29f07 antibody, mthfd1 antibody, wu:fc29f07 antibody, zgc:55597 antibody, methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 antibody, C-1-tetrahydrofolate synthase, cytoplasmic antibody, putative C-1-tetrahydrofolate synthase, cytoplasmic antibody, putative C-1-tetrahydrofolate synthase,cytoplasmic antibody, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like antibody, methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase antibody, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1b antibody, c-1-tetrahydrofolate synthase, cytoplasmic antibody, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase L homeolog antibody, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase antibody, MTHFD1 antibody, Mthfd1 antibody, Tc00.1047053509509.20 antibody, Tc00.1047053511741.50 antibody, Tc00.1047053511517.60 antibody, Tc00.1047053508207.240 antibody, Tb927.7.1600 antibody, LINJ_26_0310 antibody, LBRM_26_0350 antibody, LMJF_26_0320 antibody, mthfd1l antibody, LOC550749 antibody, mthfd1b antibody, Tsp_00651 antibody, mthfd1.L antibody, mthfd1 antibody, LOC589933 antibody, LOC100634307 antibody

Background: MTHFD1 possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MTHFC, MTHFD
Protein Interaction Partner: UBC, FUS, SUMO2, SUMO3, NEDD8, MDM2, RPA3, RPA2, RPA1, RNF2, RPS21, MAT2A, UBQLN1, FBXO6, UBD, ADRB2, CD81, VCAM1, HSP90AA1, HSP90AB1, PRKCDBP, SFXN1, TMEM165, IARS2, SARS2, SF3B3, FAF2, PPT2, SNRNP70, RPL32, MRPL23, ALDH18A1, PRPH, PC, ICT1, GAA, ACSL3,
Protein Size: 935

Molecular Weight: 101 kDa

Gene ID: 4522

NCBI Accession: NM_005956, NP_005947

UniProt: P11586

Pathways: Methionine Biosynthetic Process