FXYD1 antibody (N-Term)

Catalog No. ABIN2782662

The Rabbit Polyclonal anti-FXYD1 antibody (ABIN2782662) specifically detects FXYD1 in WB. The antibody is reactive with Human, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse and Pig samples.

Quick Overview for FXYD1 antibody (N-Term) (ABIN2782662)

Target: FXYD1 (FXYD Domain Containing Ion Transport Regulator 1 (FXYD1))

Reactivity: Human, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FXYD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FXYD1 Antibody

Binding Specificity: N-Term

Purpose: FXYD1 Antibody - N-terminal region

Sequence: ASLGHILVFC VGLLTMAKAE SPKEHDPFTY DYQSLQIGGL VIAGILFILG

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 85%, Horse: 86%, Human: 100%, Pig: 86%, Rabbit: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against FXYD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FXYD1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46562-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for FXYD1

Target: FXYD1 (FXYD Domain Containing Ion Transport Regulator 1 (FXYD1))

Alternative Name: FXYD1

Background:

Background Information: FXYD1 is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature.This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature.

Gene Name: FXYD domain containing ion transport regulator 1

Alternative Symbols: PLM

Protein Name: Phospholemman

Molecular Weight: 10kDa

Gene ID: 5348

NCBI Accession: NP_005022

UniProt: O00168