NNT antibody (N-Term)

Catalog No. ABIN2782785

Cited in 1 publication. The Rabbit Polyclonal anti-NNT antibody (ABIN2782785) specifically detects NNT in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit and Dog samples.

Quick Overview for NNT antibody (N-Term) (ABIN2782785)

Target: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NNT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NNT Antibody

Binding Specificity: N-Term

Purpose: NNT Antibody - N-terminal region

Sequence: IVRGFDTRAA ALEQFKSLGA EPLEVDLKES GEGQGGYAKE MSKEFIEAEM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NNT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NNT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46892-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NNT antibody (ABIN2782785)

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for NNT

Target: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Alternative Name: NNT

Background:

Background Information: NNT is an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.

Gene Name: Nicotinamide nucleotide transhydrogenase

Alternative Symbols: GCCD4

Protein Name: NAD(P) transhydrogenase, mitochondrial

Molecular Weight: 114kDa

Gene ID: 23530

NCBI Accession: NP_036475

UniProt: Q13423

Pathways: Proton Transport