SIGLEC7 antibody (Middle Region)

Catalog No. ABIN2782809

This Rabbit Polyclonal antibody specifically detects SIGLEC7 in WB. It exhibits reactivity toward Human, Cow, Dog, Guinea Pig and Horse.It has been mentioned in 1 publication

Quick Overview for SIGLEC7 antibody (Middle Region) (ABIN2782809)

Target: SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))

Reactivity: Human, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIGLEC7 Antibody

Binding Specificity: Middle Region

Purpose: SIGLEC7 Antibody - middle region

Sequence: WRSLTLYPSQ PSNPLVLELQ VHLGDEGEFT CRAQNSLGSQ HVSLNLSLQQ

Predicted Reactivity: Cow: 85%, Dog: 100%, Guinea Pig: 86%, Horse: 92%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against SIGLEC7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SIGLEC7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47018-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SIGLEC7 antibody (ABIN2782809)

Jaroenpool, Rogers, Pattanapanyasat, Villinger, Onlamoon, Crocker, Ansari: "Differences in the constitutive and SIV infection induced expression of Siglecs by hematopoietic cells from non-human primates." in: Cellular immunology, Vol. 250, Issue 1-2, pp. 91-104, (2008) (PubMed).

Target Details for SIGLEC7

Target: SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))

Alternative Name: SIGLEC7

Background:

Background Information: SIGLEC7 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. In the immune response, it may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. SIGLEC7 mediates inhibition of natural killer cells cytotoxicity. It may also play a role in hemopoiesis. SIGLEC7 inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).

Gene Name: Sialic acid binding Ig-like lectin 7

Alternative Symbols: p75, QA79, AIRM1, CD328, AIRM-1, CDw328, D-siglec, SIGLEC-7, SIGLECP2, SIGLEC19P, p75/AIRM1

Protein Name: Sialic acid-binding Ig-like lectin 7

Molecular Weight: 51kDa

Gene ID: 27036

NCBI Accession: NP_055200

UniProt: Q9Y286