Selenoprotein S antibody (Middle Region)

Catalog No. ABIN2783218

Cited in 3+ publications. The Rabbit Polyclonal anti-Selenoprotein S antibody (ABIN2783218) specifically detects Selenoprotein S in WB. The antibody is reactive with Human, Rat, Mouse, Dog, Cow, Goat, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae and Zebrafish (Danio rerio) samples.

Quick Overview for Selenoprotein S antibody (Middle Region) (ABIN2783218)

Target: Selenoprotein S (SELS)

Reactivity: Human, Rat, Mouse, Dog, Cow, Goat, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Selenoprotein S antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Selenoprotein S Antibody

Binding Specificity: Middle Region

Purpose: SELENOS Antibody - middle region

Sequence: PDVVVKRQEA LAAARLKMQE ELNAQVEKHK EKLKQLEEEK RRQKIEMWDS

Predicted Reactivity: Cow: 93%, Dog: 86%, Goat: 75%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 92%, Rat: 93%, Yeast: 90%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SELS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SELS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48082-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Selenoprotein S antibody (ABIN2783218)

Du, Liu, Huang: "Influence of SelS gene silence on beta-Mercaptoethanol-mediated endoplasmic reticulum stress and cell apoptosis in HepG2 cells." in: Biochimica et biophysica acta, Vol. 1800, Issue 5, pp. 511-7, (2010) (PubMed).

Moses, Johnson, Tømmerdal, Forsmo, Curran, Abraham, Charlesworth, Brennecke, Blangero, Austgulen: "Genetic association of preeclampsia to the inflammatory response gene SEPS1." in: American journal of obstetrics and gynecology, Vol. 198, Issue 3, pp. 336.e1-5, (2008) (PubMed).

Zeng, Du, Zhou, Huang: "Role of SelS in lipopolysaccharide-induced inflammatory response in hepatoma HepG2 cells." in: Archives of biochemistry and biophysics, Vol. 478, Issue 1, pp. 1-6, (2008) (PubMed).

Target Details for Selenoprotein S

Target: Selenoprotein S (SELS)

Alternative Name: SELENOS

Background:

Background Information: This gene encodes a transmembrane protein that is localized in the endoplasmic reticulum (ER). It is involved in the degradation process of misfolded proteins in the ER, and may also have a role in inflammation control. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Two additional phylogenetically conserved stem-loop structures (Stem-loop 1 and Stem-loop 2) in the 3' UTR of this mRNA have been shown to function as modulators of Sec insertion. An alternatively spliced transcript variant, lacking the SECIS element and encoding a non-Sec containing shorter isoform, has been described for this gene (PMID:23614019).

Gene Name: selenoprotein S

Alternative Symbols: SELS, VIMP, ADO15, SBBI8, SEPS1, AD-015

Protein Name: selenoprotein S

Molecular Weight: 21kDa

Gene ID: 55829

NCBI Accession: NP_060915

UniProt: Q9BQE4

Pathways: Cellular Response to Molecule of Bacterial Origin, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome