Sep 15 antibody (Middle Region)

Catalog No. ABIN2783300

This Rabbit Polyclonal antibody specifically detects Sep 15 in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig, Zebrafish (Danio rerio) and Cow.

Quick Overview for Sep 15 antibody (Middle Region) (ABIN2783300)

Target: Sep 15 (SEP15) (15 KDa Selenoprotein (SEP15))

Reactivity: Human, Mouse, Rat, Pig, Zebrafish (Danio rerio), Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sep 15 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Sep 15 Antibody

Binding Specificity: Middle Region

Sequence: SDKPKLFRGL QIKYVRGSDP VLKLLDDNGN IAEELSILKW NTDSVEEFLS

Predicted Reactivity: Cow: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SEP15. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SEP15

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 165 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Sep 15

Target: Sep 15 (SEP15) (15 KDa Selenoprotein (SEP15))

Alternative Name: SEP15

Background: SEP15 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. Studies in mouse suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. The gene that encodes the protein is localized on chromosome 1p31, a genetic locus commonly mutated or deleted in human cancers. This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Studies in mouse suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. This gene is localized on chromosome 1p31, a genetic locus commonly mutated or deleted in human cancers. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: -
Protein Interaction Partner: METTL23, UBC, DCUN1D1, CUL3, BAMBI, XRCC6, UGGT2, UGGT1,
Protein Size: 165

Molecular Weight: 15 kDa

Gene ID: 9403

NCBI Accession: NM_004261, NP_004252

UniProt: O60613