PYGB antibody (N-Term)

Catalog No. ABIN2783431

The Rabbit Polyclonal anti-PYGB antibody is suitable to detect PYGB in samples from Human, Mouse, Rat, Rabbit, Sheep, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig and Horse. It has been validated for WB and IHC.

Quick Overview for PYGB antibody (N-Term) (ABIN2783431)

Target: PYGB (GPBB) (phosphorylase, Glycogen, Brain (GPBB))

Reactivity: Human, Mouse, Rat, Rabbit, Sheep, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYGB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PYGB Antibody

Binding Specificity: N-Term

Purpose: PYGB Antibody - N-terminal region

Sequence: QQHYYERDPK RIYYLSLEFY MGRTLQNTMV NLGLQNACDE AIYQLGLDLE

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against PYGB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PYGB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48586-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PYGB

Target: PYGB (GPBB) (phosphorylase, Glycogen, Brain (GPBB))

Alternative Name: PYGB

Background:

Background Information: PYGB is a glycogen phosphorylase found predominantly in the brain. It forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Phosphorylase, glycogen, brain

Alternative Symbols: GPBB

Protein Name: Glycogen phosphorylase, brain form

Molecular Weight: 97 kDa

Gene ID: 5834

NCBI Accession: NP_002853

UniProt: P11216

Pathways: Cellular Glucan Metabolic Process