MAP3K2 antibody (N-Term)

Catalog No. ABIN503027

The Rabbit Polyclonal anti-MAP3K2 antibody is suitable to detect MAP3K2 in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Pig and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for MAP3K2 antibody (N-Term) (ABIN503027)

Target: MAP3K2 (Mitogen-Activated Protein Kinase Kinase Kinase 2 (MAP3K2))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP3K2 Antibody

Binding Specificity: N-Term

Purpose: MAP3K2 Antibody - N-terminal region

Sequence: AERKKRLSII GPTSRDRSSP PPGYIPDELH QVARNGSFTS INSEGEFIPE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 80%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 93%, Rat: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MAP3K2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MAP3K2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP49223-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MAP3K2

Target: MAP3K2 (Mitogen-Activated Protein Kinase Kinase Kinase 2 (MAP3K2))

Alternative Name: MAP3K2

Background:

Background Information: MAP3K2 is a component of a protein kinase signal transduction cascade. It regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7. It also plays a role in caveolae kiss-and-run dynamics.The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-18 AF111105.1 37-54 19-79 AI760702.1 474-534 80-371 AF111105.1 116-407 372-2650 AB208963.1 313-2591 2651-3557 AK075004.1 2050-2956 3558-3571 BQ423724.1 71-84 3572-3577 W07290.1 11-16 3578-4302 AK074577.1 1-725 4303-4421 BM470563.1 21-139 4422-4424 AK074577.1 845-847 4425-4941 BM470563.1 142-658

Gene Name: Mitogen-activated protein kinase kinase kinase 2

Alternative Symbols: MEKK2, MEKK2B

Protein Name: Mitogen-activated protein kinase kinase kinase 2

Molecular Weight: 70kDa

Gene ID: 10746

NCBI Accession: NP_006600

UniProt: Q9Y2U5

Pathways: MAPK Signaling