FHIT antibody (Middle Region)

Catalog No. ABIN2784442

This Rabbit Polyclonal antibody specifically detects FHIT in WB. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Horse, Zebrafish (Danio rerio), Dog and Rabbit.

Quick Overview for FHIT antibody (Middle Region) (ABIN2784442)

Target: FHIT (Fragile Histidine Triad (FHIT))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Zebrafish (Danio rerio), Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHIT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FHIT Antibody

Binding Specificity: Middle Region

Sequence: VHVLPRKAGD FHRNDSIYEE LQKHDKEDFP ASWRSEEEMA AEAAALRVYF

Predicted Reactivity: Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Rabbit: 93%, Rat: 79%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against FHIT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FHIT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 147 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FHIT

Target: FHIT (Fragile Histidine Triad (FHIT))

Alternative Name: FHIT

Background: This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene.
Alias Symbols: AP3Aase, FRA3B
Protein Interaction Partner: FHIT, REL, MDM2, UBC, RAB40B, TRIM23, LEF1, CTNNB1, UBE2I, SRC, GSN, HSPD1, FDXR,
Protein Size: 147

Molecular Weight: 17 kDa

Gene ID: 2272

NCBI Accession: NM_002012, NP_002003

UniProt: P49789