FHIT antibody (AA 1-147)

Catalog No. ABIN3042392

There are 2+ publications for this product available. The Rabbit Polyclonal anti-FHIT antibody is suitable to detect FHIT in samples from Human and Rat. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for FHIT antibody (AA 1-147) (ABIN3042392)

Target: FHIT (Fragile Histidine Triad (FHIT))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHIT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-FHIT Antibody

Binding Specificity: AA 1-147

Purpose: Anti-FHIT Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human FHIT shares 90%,87% amino acid (aa) sequences identity with mouse,rat FHIT,respectively.

Characteristics: Anti-FHIT Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FHIT recombinant protein (Position: M1-Q147). Human FHIT shares 90% and 87% amino acid (aa) sequences identity with mouse and rat FHIT, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-FHIT antibody (ABIN3042392)

Li, Li, Xu, Guo, Du: "Neuroprotective properties of picroside II in a rat model of focal cerebral ischemia." in: International journal of molecular sciences, Vol. 11, Issue 11, pp. 4580-90, (2010) (PubMed).

Tracy, Liu, Chapman: "Coxsackievirus B3: primary structure of the 5' non-coding and capsid protein-coding regions of the genome." in: Virus research, Vol. 3, Issue 3, pp. 263-70, (1986) (PubMed).

Target Details for FHIT

Target: FHIT (Fragile Histidine Triad (FHIT))

Alternative Name: FHIT

Background:

Background: Bis (5'-adenosyl)-triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1,P3-bis (5'-adenosyl)-triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.

Gene Full Name: fragile histidine triad diadenosine triphosphatase

Sequence Similarities: Contains 1 HIT domain.

Molecular Weight: 17 kDa

Gene ID: 2272

UniProt: P49789