MAP1LC3A antibody (N-Term)

Catalog No. ABIN2784522

This Rabbit Polyclonal antibody specifically detects MAP1LC3A in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse and Dog.It has been mentioned in 1 publication

Quick Overview for MAP1LC3A antibody (N-Term) (ABIN2784522)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MAP1LC3A Antibody

Binding Specificity: N-Term

Purpose: MAP1LC3A Antibody - N-terminal region

Sequence: MKMRFFSSPC GKAAVDPADR CKEVQQIRDQ HPSKIPVIIE RYKGEKQLPV

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against MAP1LC3A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MAP1LC3A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51335-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAP1LC3A antibody (ABIN2784522)

Taylor, Kirkegaard: "Modification of cellular autophagy protein LC3 by poliovirus." in: Journal of virology, Vol. 81, Issue 22, pp. 12543-53, (2007) (PubMed).

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: MAP1LC3A

Background:

Background Information: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B.MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: Microtubule-associated protein 1 light chain 3 alpha

Alternative Symbols: LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3

Protein Name: Microtubule-associated proteins 1A/1B light chain 3A

Molecular Weight: 14kDa

Gene ID: 84557

NCBI Accession: NP_852610

UniProt: Q9H492

Pathways: Autophagy