PPP3CA antibody (Middle Region)

Catalog No. ABIN2784524

The Rabbit Polyclonal anti-PPP3CA antibody (ABIN2784524) specifically detects PPP3CA in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Zebrafish (Danio rerio) and Guinea Pig samples.

Quick Overview for PPP3CA antibody (Middle Region) (ABIN2784524)

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP3CA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPP3CA Antibody

Binding Specificity: Middle Region

Sequence: LPSGVLSGGK QTLQSATVEA IEADEAIKGF SPQHKITSFE EAKGLDRINE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against PPP3CA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPP3CA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 521 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPP3CA

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Alternative Name: PPP3CA

Background: PPP3CA belongs to the PPP phosphatase family, PP-2B subfamily. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. PPP3CA dephosphorylates HSPB1 and SSH1.
Alias Symbols: CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B
Protein Interaction Partner: C16orf74, PPP3R1, JUN, CEP76, MARCH7, VCP, TP53, UBC, SETSIP, P3H1, XPO5, MAT2B, TNPO3, PDIA6, VBP1, TSN, PPP2R2A, PFDN5, NRD1, NASP, TNPO1, HNRNPH1, ALY1, MAPK7, RCAN1, MRI1, RHOC, TRAF3, KCNIP3, PDE4D, PSMA6, PHLPP2, PTEN, AKT1, IRF2, GRB2, Fbxo32, MYOZ
Protein Size: 521

Molecular Weight: 59 kDa

Gene ID: 5530

NCBI Accession: NM_000944, NP_000935

UniProt: Q08209

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling