DPH1 antibody (Middle Region)

Catalog No. ABIN2784708

The Rabbit Polyclonal anti-DPH1 antibody is suitable to detect DPH1 in samples from Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Cow, Saccharomyces cerevisiae and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for DPH1 antibody (Middle Region) (ABIN2784708)

Target: DPH1 (DPH1 Homolog (DPH1))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Cow, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DPH1 Antibody

Binding Specificity: Middle Region

Purpose: DPH1 Antibody - middle region

Sequence: RMQAARQEAI ATARSAKSWG LILGTLGRQG SPKILEHLES RLRALGLSFV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against DPH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DPH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099206

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DPH1

Target: DPH1 (DPH1 Homolog (DPH1))

Alternative Name: DPH1

Background:

Background Information: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-40 AF321876.1 1-40 41-1028 BC003099.1 3-990 1029-1410 BP417755.1 78-459 1411-2188 AK090530.1 1484-2261 2189-2200 AF321876.1 2189-2200

Gene Name: DPH1 homolog (S. cerevisiae)

Alternative Symbols: DPH2L, OVCA1, DEDSSH, DPH2L1

Protein Name: Diphthamide biosynthesis protein 1

Molecular Weight: 49 kDa

Gene ID: 1801

NCBI Accession: NP_001374

UniProt: Q9BZG8