ACTR1B antibody (Middle Region)

Catalog No. ABIN2784772

The Rabbit Polyclonal anti-ACTR1B antibody is suitable to detect ACTR1B in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for ACTR1B antibody (Middle Region) (ABIN2784772)

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTR1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACTR1B Antibody

Binding Specificity: Middle Region

Sequence: VGPARFRAPE LLFQPDLVGD ESEGLHEVVA FAIHKSDMDL RRTLFANIVL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 77%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ACTR1B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ACTR1B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 376 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ACTR1B

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Alternative Name: ACTR1B

Background: ACTR1B is a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. ACTR1B, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90 % amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex.
Alias Symbols: ARP1B, CTRN2, PC3
Protein Interaction Partner: SEH1L, ACTR10, DCTN4, DCTN2, ACTR1A, RABEP1, LMNB1, DCTN3, APP, SMAD6, CUL3, UBC, TK1, MAPK6, CSNK2B, Dctn1, Capza1, CHD3, ACTA1,
Protein Size: 376

Molecular Weight: 42 kDa

Gene ID: 10120

NCBI Accession: NM_005735, NP_005726

UniProt: P42025