PIAS2 antibody (N-Term)

Catalog No. ABIN2785610

The Rabbit Polyclonal anti-PIAS2 antibody is suitable to detect PIAS2 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Pig. It has been validated for WB.

Quick Overview for PIAS2 antibody (N-Term) (ABIN2785610)

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIAS2 Antibody

Binding Specificity: N-Term

Purpose: PIAS2 Antibody - N-terminal region

Sequence: RELYRRRYPR TLEGLSDLST IKSSVFSLDG GSSPVEPDLA VAGIHSLPST

Predicted Reactivity: Cow: 93%, Dog: 92%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Pig: 93%, Rabbit: 92%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against PIAS2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109280

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Alternative Name: PIAS2

Background:

Background Information: Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. It plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It seems to be mostly involved in gene silencing. It binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.

Gene Name: Protein inhibitor of activated STAT, 2

Alternative Symbols: DIP, MIZ1, SIZ2, ARIP3, PIASX, ZMIZ4

Protein Name: E3 SUMO-protein ligase PIAS2

Molecular Weight: 63kDa

Gene ID: 9063

NCBI Accession: NP_775298

UniProt: O75928

Pathways: JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling