ETFA antibody (N-Term)

Catalog No. ABIN2785636

This Rabbit Polyclonal antibody specifically detects ETFA in WB. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow and Dog.

Quick Overview for ETFA antibody (N-Term) (ABIN2785636)

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETFA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ETFA Antibody

Binding Specificity: N-Term

Purpose: ETFA Antibody - N-terminal region

Sequence: FRAAAPGQLR RAASLLRFQS TLVIAEHAND SLAPITLNTI TAATRLGGEV

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ETFA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ETFA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54289-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ETFA

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Alternative Name: ETFA

Background:

Targeted Subunit: alpha, mitochondrial

Background Information: ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: Electron-transfer-flavoprotein, alpha polypeptide

Alternative Symbols: EMA, GA2, MADD

Protein Name: Electron transfer flavoprotein subunit alpha, mitochondrial

Molecular Weight: 35kDa

Gene ID: 2108

NCBI Accession: NP_000117

UniProt: P13804

Pathways: SARS-CoV-2 Protein Interactome