Hexokinase 2 antibody (Middle Region)

Catalog No. ABIN2785643

The Rabbit Polyclonal anti-Hexokinase 2 antibody is suitable to detect Hexokinase 2 in samples from Human, Mouse, Rat, Pig, Dog, Cow, Horse, Rabbit and Guinea Pig. It has been validated for WB.

Quick Overview for Hexokinase 2 antibody (Middle Region) (ABIN2785643)

Target: Hexokinase 2 (HK2)

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Hexokinase 2 Antibody

Binding Specificity: Middle Region

Purpose: HK2 Antibody - middle region

Sequence: QRIKENKGEE RLRSTIGVDG SVYKKHPHFA KRLHKTVRRL VPGCDVRFLR

Predicted Reactivity: Cow: 79%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HK2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HK2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54304-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: HK2

Background:

Background Information: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Gene Name: Hexokinase 2

Alternative Symbols: HKII, HXK2

Protein Name: Hexokinase-2

Molecular Weight: 102kDa

Gene ID: 3099

NCBI Accession: NP_000180

UniProt: P52789

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect