LIG1 antibody (Middle Region)

Catalog No. ABIN2785646

The Rabbit Polyclonal anti-LIG1 antibody (ABIN2785646) specifically detects LIG1 in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Rabbit, Dog, Horse, Guinea Pig, Goat and Zebrafish (Danio rerio) samples.

Quick Overview for LIG1 antibody (Middle Region) (ABIN2785646)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Dog, Horse, Guinea Pig, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIG1 Antibody

Binding Specificity: Middle Region

Purpose: LIG1 Antibody - middle region

Sequence: ALEGGEVKIF SRNQEDNTGK YPDIISRIPK IKLPSVTSFI LDTEAVAWDR

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 79%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against LIG1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human LIG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54307-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1

Background:

Background Information: This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Gene Name: DNA ligase 1

Protein Name: DNA ligase 1

Molecular Weight: 102kDa

Gene ID: 3978

NCBI Accession: NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA