Granulin antibody (Middle Region)

Catalog No. ABIN2785816

This Rabbit Polyclonal antibody specifically detects Granulin in WB and IHC. It exhibits reactivity toward Human, Horse, Cow, Dog and Rabbit.

Quick Overview for Granulin antibody (Middle Region) (ABIN2785816)

Target: Granulin (GRN)

Reactivity: Human, Horse, Cow, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Granulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Granulin Antibody

Binding Specificity: Middle Region

Purpose: GRN Antibody - middle region

Sequence: CPMPNATCCS DHLHCCPQDT VCDLIQSKCL SKENATTDLL TKLPAHTVGD

Predicted Reactivity: Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Rabbit: 85%

Characteristics: This is a rabbit polyclonal antibody against GRN. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human GRN

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54641-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Granulin

Target: Granulin (GRN)

Alternative Name: GRN

Background:

Background Information: Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Gene Name: granulin

Alternative Symbols: GEP, GP88, PEPI, PGRN, CLN11, PCDGF

Protein Name: Granulins

Molecular Weight: 47 kDa

Gene ID: 2896

NCBI Accession: NP_002078

UniProt: P28799