RHOB antibody (Middle Region)

Catalog No. ABIN2785839

The Rabbit Polyclonal anti-RHOB antibody (ABIN2785839) specifically detects RHOB in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Goat, Horse, Pig, Sheep, Dog, Guinea Pig, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for RHOB antibody (Middle Region) (ABIN2785839)

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Reactivity: Human, Mouse, Rat, Cow, Goat, Horse, Pig, Sheep, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RHOB Antibody

Binding Specificity: Middle Region

Purpose: RHOB Antibody - middle region

Sequence: CPNVPIILVA NKKDLRSDEH VRTELARMKQ EPVRTDDGRA MAVRIQAYDY

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 79%, Rat: 100%, Sheep: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against RHOB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RHOB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54702-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RHOB

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Alternative Name: RHOB

Background:

Background Information: RHOB mediates apoptosis in neoplastically transformed cells after DNA damage.RHOB is not essential for development but affects cell adhesion and growth factor signaling in transformed cells.RHOB plays a negative role in tumorigenesis as deletion causes tumor formation. RHOB is involved in intracellular protein trafficking of a number of proteins.RHOB targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes.RHOB is also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.

Gene Name: Ras homolog family member B

Alternative Symbols: ARH6, ARHB, RHOH6, MST081, MSTP081

Protein Name: Rho-related GTP-binding protein RhoB

Molecular Weight: 22kDa

Gene ID: 388

NCBI Accession: NP_004031

UniProt: P62745