14-3-3 sigma/SFN antibody (N-Term)

Catalog No. ABIN2785885

The Rabbit Polyclonal anti-14-3-3 sigma/SFN antibody is suitable to detect 14-3-3 sigma/SFN in samples from Human, Mouse, Rat, Cow, Dog, Sheep, Horse, Rabbit and Guinea Pig. It has been validated for WB.

Quick Overview for 14-3-3 sigma/SFN antibody (N-Term) (ABIN2785885)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: N-Term

Purpose: SFN Antibody - N-terminal region

Sequence: VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against SFN. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SFN

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54791-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN

Background:

Background Information: SFN is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. SFN binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, SFN regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.

Gene Name: Stratifin

Alternative Symbols: YWHAS

Protein Name: 14-3-3 protein sigma

Molecular Weight: 28kDa

Gene ID: 2810

NCBI Accession: NP_006133

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction