POLL antibody (Middle Region)

Catalog No. ABIN2785955

This Rabbit Polyclonal antibody specifically detects POLL in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Dog and Saccharomyces cerevisiae.

Quick Overview for POLL antibody (Middle Region) (ABIN2785955)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Dog, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLL Antibody

Binding Specificity: Middle Region

Purpose: POLL Antibody - middle region

Sequence: SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%, Yeast: 83%

Characteristics: This is a rabbit polyclonal antibody against POLL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54924-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Alternative Name: POLL

Background:

Background Information: POLL is a repair polymerase.It is involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.

Gene Name: Polymerase (DNA directed), lambda

Alternative Symbols: BETAN, POLKAPPA

Protein Name: DNA polymerase lambda

Molecular Weight: 63kDa

Gene ID: 27343

NCBI Accession: NP_037406

UniProt: Q9UGP5

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response