POLM antibody (Middle Region)

Catalog No. ABIN406451

The Rabbit Polyclonal anti-POLM antibody is suitable to detect POLM in samples from Human, Mouse, Rat, Horse, Pig, Rabbit, Cow, Dog and Guinea Pig. It has been validated for WB.

Quick Overview for POLM antibody (Middle Region) (ABIN406451)

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Reactivity: Human, Mouse, Rat, Horse, Pig, Rabbit, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLM Antibody

Binding Specificity: Middle Region

Purpose: POLM Antibody - middle region

Sequence: LRRFSRKEKG LWLNSHGLFD PEQKTFFQAA SEEDIFRHLG LEYLPPEQRN

Predicted Reactivity: Cow: 79%, Dog: 92%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against POLM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54928-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLM

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Alternative Name: POLM

Background:

Background Information: POLM seems to act as an Ig mutase which is responsible for immunoglobulin (Ig) gene hypermutation.

Gene Name: Polymerase (DNA directed), mu

Alternative Symbols: Tdt-N, Pol Mu

Protein Name: DNA-directed DNA/RNA polymerase mu

Molecular Weight: 55kDa

Gene ID: 27434

NCBI Accession: NP_037416

UniProt: Q9NP87

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response