RHOC antibody (N-Term)

Catalog No. ABIN2786344

Cited in 1 publication. The Rabbit Polyclonal anti-RHOC antibody (ABIN2786344) specifically detects RHOC in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio), Saccharomyces cerevisiae, Sheep and Goat samples.

Quick Overview for RHOC antibody (N-Term) (ABIN2786344)

Target: RHOC (Ras Homolog Gene Family, Member C (RHOC))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio), Saccharomyces cerevisiae, Sheep, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RHOC Antibody

Binding Specificity: N-Term

Purpose: RHOC Antibody - N-terminal region

Sequence: VPTVFENYIA DIEVDGKQVE LALWDTAGQE DYDRLRPLSY PDTDVILMCF

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RHOC. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RHOC

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55742-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RHOC antibody (ABIN2786344)

Sousa, Espreafico: "Suppression subtractive hybridization profiles of radial growth phase and metastatic melanoma cell lines reveal novel potential targets." in: BMC cancer, Vol. 8, pp. 19, (2008) (PubMed).

Target Details for RHOC

Target: RHOC (Ras Homolog Gene Family, Member C (RHOC))

Alternative Name: RHOC

Background:

Background Information: RHOC Is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. RHOC is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of RHOC is associated with tumor cell proliferation and metastasis.This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gene Name: Ras homolog family member C

Alternative Symbols: H9, ARH9, ARHC, RHOH9

Protein Name: Rho-related GTP-binding protein RhoC

Molecular Weight: 22kDa

Gene ID: 389

NCBI Accession: NP_786886

UniProt: P08134

Pathways: WNT Signaling, Cell-Cell Junction Organization