ERK1 antibody (Middle Region)

Catalog No. ABIN2786698

This Rabbit Polyclonal antibody specifically detects ERK1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Dog, Cow, Zebrafish (Danio rerio), Horse, Guinea Pig and Goat.

Quick Overview for ERK1 antibody (Middle Region) (ABIN2786698)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Zebrafish (Danio rerio), Horse, Guinea Pig, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERK1 Antibody

Binding Specificity: Middle Region

Sequence: LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 79%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against MAPK3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MAPK3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 379 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, a
Alias Symbols: ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3, ERT2, ERK-1, p44-ERK1, p44-MAPK
Protein Interaction Partner: MAPK1, DUSP4, STAR, RAF1, PTGDR, MAP2K1, NAB2, MYOG, DHPS, HDAC6, MAGED1, AMOT, FOXP2, CDC23, VPS52, L3MBTL3, TRIM54, DCP1A, UBC, TP53, PTGS2, RPS6KA2, MAPK3, PHF3, TEK, MBP, ELK1, CUEDC2, MAPKAPK2, GAB2, IRX2, ADAM15, VCP, ELK4, DAPK1, Rara, IQGAP1, TP53
Protein Size: 379

Molecular Weight: 43 kDa

Gene ID: 5595

NCBI Accession: NM_002746, NP_002737

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins