GBL antibody (Middle Region)

Catalog No. ABIN2787309

This Rabbit Polyclonal antibody specifically detects GBL in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Rabbit, Horse and Zebrafish (Danio rerio).

Quick Overview for GBL antibody (Middle Region) (ABIN2787309)

Target: GBL (G protein beta subunit-like (GBL))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GBL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GBL Antibody

Binding Specificity: Middle Region

Purpose: GBL Antibody - middle region

Sequence: CAFSGDSQYI VTASSDNLAR LWCVETGEIK REYGGHQKAV VCLAFNDSVL

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against GBL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GBL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57630-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GBL

Target: GBL (G protein beta subunit-like (GBL))

Alternative Name: GBL

Background:

Targeted Subunit: LST8

Background Information: GBL is the subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Gene Name: MTOR associated protein, LST8 homolog (S. cerevisiae)

Alternative Symbols: GBL, LST8, POP3, WAT1, GbetaL

Protein Name: Target of rapamycin complex subunit LST8

Molecular Weight: 36kDa

Gene ID: 64223

NCBI Accession: NP_071767

UniProt: Q9BVC4

Pathways: PI3K-Akt Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect