Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (Middle Region) antibody

Details for Product No. ABIN2787377
Antigen
  • Ph-4
  • RGD1311848
  • EGLN4
  • HIFPH4
  • P4H-TM
  • PH-4
  • PH4
  • PHD4
  • 4933406E20Rik
  • AI853847
  • BB128974
  • prolyl 4-hydroxylase, transmembrane
  • prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
  • shisa family member 5
  • P4htm
  • P4HTM
  • SHISA5
Epitope
Middle Region
4
4
3
3
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
63
48
37
3
3
3
3
2
2
2
2
1
Host
Rabbit
62
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
3
3
3
3
3
3
2
2
2
2
2
Application
Western Blotting (WB)
26
26
6
6
4
3
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PH-4
Blocking Peptide Blocking peptide for this product available: ABIN981656
Sequence RLGNGWWMTP ESIQEMYAAI KADPDGDGVL SLQEFSNMDL RDFHKYMRSH
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against PH-4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Antigen
Alternative Name PH-4 (P4HTM Antibody Abstract)
Background The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and m
Alias Symbols: P4H-TM, PH4, PH-4, PHD4, EGLN4, HIFPH4
Protein Interaction Partner: GLP1R, PDIA2, nef, UBC, CD1D,
Protein Size: 502
Molecular Weight 57 kDa
Gene ID 54681
NCBI Accession NM_177939, NP_808808
UniProt Q9NXG6
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 502 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (Middle Region) antibody (ABIN2787377) WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive ...
Background publications Koivunen, Tiainen, Hyvärinen, Williams, Sormunen, Klaus, Kivirikko, Myllyharju: "An endoplasmic reticulum transmembrane prolyl 4-hydroxylase is induced by hypoxia and acts on hypoxia-inducible factor alpha." in: The Journal of biological chemistry, Vol. 282, Issue 42, pp. 30544-52, 2007 (PubMed).

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