PRKACA antibody (N-Term)

Catalog No. ABIN2787416

The Rabbit Polyclonal anti-PRKACA antibody is suitable to detect PRKACA in samples from Human, Mouse, Rat, Dog, Cow, Sheep, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Pig. It has been validated for WB and IHC.

Quick Overview for PRKACA antibody (N-Term) (ABIN2787416)

Target: PRKACA (Protein Kinase A, alpha (PRKACA))

Reactivity: Human, Mouse, Rat, Dog, Cow, Sheep, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKACA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRKACA Antibody

Binding Specificity: N-Term

Purpose: PRKACA Antibody - N-terminal region

Sequence: MASNSSDVKE FLAKAKEDFL KKWESPAQNT AHLDQFERIK TLGTGSFGRV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%, Sheep: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against PRKACA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRKACA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57828-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRKACA

Target: PRKACA (Protein Kinase A, alpha (PRKACA))

Alternative Name: PRKACA

Background:

Background Information: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene Name: Protein kinase, cAMP-dependent, catalytic, alpha

Alternative Symbols: CAFD1, PKACA, PPNAD4

Protein Name: cDNA FLJ77368, highly similar to Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA EMBL BAF84973.1

Molecular Weight: 40

Gene ID: 5566

NCBI Accession: NP_997401

UniProt: A8K8B9

Pathways: NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection