APEX1 antibody (Middle Region)

Catalog No. ABIN2787519

The Rabbit Polyclonal anti-APEX1 antibody (ABIN2787519) specifically detects APEX1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig, Goat and Zebrafish (Danio rerio) samples.

Quick Overview for APEX1 antibody (Middle Region) (ABIN2787519)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-APEX1 Antibody

Binding Specificity: Middle Region

Purpose: APEX1 Antibody - middle region

Sequence: HEEIDLRNPK GNKKNAGFTP QERQGFGELL QAVPLADSFR HLYPNTPYAY

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against APEX1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human APEX1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58028-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1

Background:

Background Information: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene, all encode the same protein.

Gene Name: APEX nuclease (multifunctional DNA repair enzyme) 1

Alternative Symbols: APE, APX, APE1, APEN, APEX, HAP1, REF1

Protein Name: DNA-(apurinic or apyrimidinic site) lyase

Molecular Weight: 36 kDa

Gene ID: 328

NCBI Accession: NP_001632

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus